IRIS
Journal for Young Scientists
ISSN 2278-618X (Print)
ISSN 2278-6384 (Online)
© Patna Women’s College, Patna, India
Formulation of Herbal Detergent and study of its efficacy against selected microbes
• Urvashi Sinha • Kriti Kumari • Kumkum Kant • Mansi Srivastava
Received : January, 2024
Accepted : February, 2024
Corresponding Author : Urvashi Sinha
Abstract: Formulation of the herbal detergent was done using berries of Sapindus mukorossi (Soapnut), fruits of Citrus limon (lemon), fruits of Citrus sinesis (Orange) along with leaves of Azadirachta indica (Neem) and leaves of Aloe barbadensis miller (Aloe vera). The effectiveness of freshly prepared herbal detergent was evaluated on the basis of colour, odour, homogeneity and pH with the outcome at regular interval of 15 days and 30 days. The colour of the herbal detergent changed slightly from off white to clear white and odour was found to be pleasant for the freshly prepared sample and strong odour was observed after 30 days with consistent homogeneity and the herbal detergent was found very effective against hard stains (turmeric, oil, etc.) and light stains (tea, coffee, etc.). The colour of the pH strip was found to be dark green suggesting the pH of the herbal detergent being 7. Agar well diffusion technique was
used for assessment of antibacterial activity against Escherichia coli and Candida albicans. The plates inoculated showed varying diameter of zone of inhibition against the controls (I and II) and the test sample. In plates inoculated with Escherichia coli, the control I (20 mm) had a larger zone of inhibition than the test sample (18 ± 0.5 mm) while the control II (12 mm) had a smaller zone of inhibition than the test sample (14 ± 0.5 mm). The results of zone of inhibition against Candida albicans, of test sample (16 ± 0.5 mm, 17 ± 0.5 mm) was larger than control I (14 mm) and II (15 mm). It implies the herbal detergent to be antimicrobial, eco-friendly and effective toward different stains on various clothes.
Keywords: Surfactants, Detergent, Sapindus mukorossi, Escherichia coli, Candida albicans, Homogeneity.
Urvashi Sinha
Assistant Professor, Department of Botany,
Patna Women’s College (Autonomous),
Bailey Road, Patna–800 001, Bihar, India
E-mail : urvashi.bot@patnawomenscollege.in
Kriti Kumari
B.Sc. III year, Botany (Hons.), Session : 2021-2024,
Patna Women’s College (Autonomous),
Patna University, Patna, Bihar, India
Kumkum Kant
B.Sc. III year, Botany (Hons.), Session : 2021-2024,
Patna Women’s College (Autonomous),
Patna University, Patna, Bihar, India
Mansi Srivastava
B.Sc. III year, Botany (Hons.), Session : 2021-2024,
Patna Women’s College (Autonomous),
Patna University, Patna, Bihar, India
