Explore –Journal of Research
Peer Reviewed Journal
ISSN 2278–0297 (Print)
ISSN 2278–6414 (Online)
Vol. XVII, 2024
© Patna Women’s College, Patna, India
Bioprospecting of cellulolytic microorganisms for biodegradation and biofuel production
•Jaya Philip• Diksha Kaushik• Khushi
•Anjali Mishra
Received : December, 2023
Accepted : January, 2024
Corresponding Author : Jaya Philip
Abstract : Cellulose is a complex carbohydrate and a key structural component in the cell walls of plants. This study mainly explores the bioprospecting of cellulolytic bacteria for efficient cellulose waste degradation and subsequent biofuel production contributing to India’s vision of /ow-carbon emission. Through isolation and characterization, potential isolates were identified for their enzymatic activities crucial in breakdown of cellulose. Partial purification of enzyme was ensured by Ammonium sulphate precipitation. Enzyme assay was performed for presence of the enzyme using dinitro salicylic acid (DNS) method used for estimation of reducing sugars. Saccharomyces cerevisiae was employed in the fermentation process, resulting in the production of bio ethanol. Degradation of cellulolytic compounds leads to
bioconversion of cellulose into ethanol which is a prime biofuel. Leveraging these microorganisms not only enhances our understanding of natural cellulose degradation but also provide practical solutions for waste management through degradation of paper waste. The cellulolytic potential of such microorganisms helps in natural and cost efficient degradation of paper waste. Furthermore, the discovery of cellulolytic strains contributes to emphasizing the potential for eco friendly alternatives in the energy sector. In conclusion bioprospecting from widely existing organic material opens doors to innovative approaches in both waste management and renewable energy production.
Keywords : Cellulose, Cellulolytic bacteria, Carboxymethyl Cellulose (CMG), Paper degradation, Bioethanol.
Jaya Philip
Head, Department of Microbiology Patna Women’s College (Autonomous),
Bailey Road, Patna-800 001, Bihar, India
E-mail: jaya.mbio@patnawomenscollege.in
Diksha Kaushik
B.Sc. III year, Microbiology (Hons.), Session: 2021-2024, Patna Women’s College (Autonomous),
Patna University, Patna, Bihar, India
Khushi
B.Sc. III year, Microbiology (Hons.), Session: 2021-2024, Patna Women’s College (Autonomous),
Patna University, Patna, Bihar, India
Anjali Mishra
B.Sc. III year, Microbiology (Hons.), Session: 2021-2024, Patna Women’s College (Autonomous),
Patna University, Patna, Bihar, India
Introduction:
Cellulosic waste which is degraded by burning is a high rise global concern and thus we can effectively degrade these wastes by using cellulose degrading microorganisms by production of cellulase enzyme which are highly efficient and these are also environmentally friendly thus helping us to maintain sustainable development. Cellulose being the most abundant organic compound is a linear polysaccharide of glucose units. It can be used as a effective raw material in various industries such as textile industry, pulp and paper industry, agriculture industry, food industry for producing important products (Gupta et al., 2012). The process of breakdown of cellulose into glucose refers to as cellulolysis. Cellulase enzymes are
