Explore –Journal of Research
Peer Reviewed Journal
ISSN 2278–0297 (Print)
ISSN 2278–6414 (Online)
Vol. XVII, 2024
© Patna Women’s College, Patna, India
Extraction of essential oil from Pumpkin seeds and characterization for showing its inhibitory activity against the bacteria
•Madhu Rani Sinha• Komal• Vani Raj
•Anjali Kumari
Received : December, 2023
Accepted : January, 2024
Corresponding Author : Madhu Rani Sinha
Abstract : The essential oil extracted from pumpkin seeds have high nutritional value as it is a rich source of Zinc, Magnesium, essential amino acid and polyunsaturated fatty acids (PUFAs). This research aimed to study about the extraction of essential oils and its antibacterial application on S. aureus and E. coli. The extraction process was carried out using steam distillation and the Agar well assay method was used to show its antibacterial activity The extraction process was carried out for about 2 hours or 120 minutes with the solid particle size of 0.25mm at an optimum temperature. In Agar
well Assay method , NA media was prepared and was autoclaved along with the glass plates. The FT-IR characterization of the extracted oil showed the presence of functional groups of alkane, alkene, carbonyl, ester, amine and alcohol. In Agar well assay, the inhibitory activity of the oil sample was analysed through the zone of inhibition around the wells.
Keywords PUFAs, Steam distillation, Agar well assay, NA media, FT-IR, zone of inhibition, essential amino acid, glass plates.
Madhu Rani Sinha
Head, Department of Chemistry,
Patna Women’s College (Autonomous), Bailey Road, Patna-800 001, Bihar, India
E-mail:madhu.che@patnawomenscollege.in
Komal
B.Sc. Ill year, Chemistry (Hons.),Session: 2021-2024, Patna Women’s College (Autonomous),
Patna University, Patna, Bihar, India
Vani Raj
B.Sc. Ill year, Chemistry (Hons.), Session: 2021-2024, Patna Women’s College (Autonomous),
Patna University, Patna, Bihar, India
Anjali Kumari
B.Sc. Ill year, Chemistry (Hons.),Session: 2021-2024, Patna Women’s College (Autonomous),
Patna University, Patna, Bihar, India
Introduction:
Pumpkins are believed to have originated in central America over 7,500 years ago. The first pumpkins held away little resemblance to the sweet, bright orange variety. The original pumpkins were small and hard with a bitter flavour. Pumpkins have been a component of Indian cuisine since ancient times, they were grown on the banks of rivers on the outskirts of villages. The sap and the pulp of pumpkins has long been used as the treatment for burns. Seeds have also been used as a diuretic (any substance that promotes diuresis or production of urine). Dry strips of pumpkins were used as household mats (Herbazest, 2021). Pumpkin seeds have been eaten throughout history for subsistence and medical purposes (Jen OG, 2019).
The name pumpkin originated from the Greek word ‘PEPON’ , which means large melon (by Ranjinipinky et al, 2020). In Sanskrit, pumpkin is called as ‘KUSMANDAKAH’ @tiftl□5cb:) (Adi Parva, chapter 55
